Expression of Mipul in Response to Myocardial Infarction in Mice

Xianz Hong Xiao

Myocardial ischemic preconditioning over- regulated protein 1(Mipu1) was reproduced in our laboratory. Manly Wistar rats were subordinated to left anterior coronary roadway ligation and sham- operation and offered at 1 h, 3 h, 6 h, 12 h, 24 h, 3 d or 5 d after ligation. Expression of Mipu1 mRNA and protein were assessed by Northern blotting, real- time quantitative RT- PCR, In Situ hybridization and Western blotting. Expression of Mipu1 was over- regulated at 3 h and lasted to 12 h with a peak at 6h. Mipu1 mRNA and protein signals express in the endothelium and myocardium in normal and infarcted heart, substantially in infarcted zone. Fluorescent immunocytochemistry showed that Mipu1 protein was localized to the capitals of H9c2 myogenic cells and was up regulated after the cells being exposed toH2O2.These compliances indicates that Mipu1 may play a part in maintaining vascular homeostasis and guarding the myogenic cells from being injured by ischemiareperfusion or oxidation stress