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Improving the downstream processing of vaccine and gene therapy vectors with continuous chromatography

Ricardo JS Silva, Josa PB Mota, Cristina Peixoto, Paula M Alves and Manuel JT Carrondo*

Novel biopharmaceutical products, such as vaccines and viral vectors, are a challenging task for downstream processing. Alternative purification strategies that can improve the purification yield, such as continuous chromatography, are regarded nowadays as enabling technologies to overcome the capacity bottleneck in biomanufacturing. This paper reviews the current state of the art in (semi-)continuous chromatographic processes and equipment for purification of bioproducts. Particular focus is given to the recent multicolumn processes developed for virus purification, for which the chromatographic media selection, predictive models, and process design principles are illustrated.